hbepic cell line  (Innoprot Inc)

Journal: American Journal of Cancer Research

Article Title: Loss of MEG3 contributes to the enhanced migration and invasion in arsenic-induced carcinogenesis through NQO1/FSCN1 pathway

doi:

Figure Lengend Snippet: Arsenic-transformed cells exhibit enhanced abilities of both migration and invasion. Normal human bronchial epithelial (BEAS-2B) cells were chronically exposed to low-dose arsenic for over six months. The soft agar assay was performed and single colonies from soft agar were isolated and expanded in the culture plates. These cells were used as arsenic-transformed cells (As-T), and passage-matched normal BEAS-2B cells without arsenic exposure were used as control (As-M). (A) Cell migration and invasion were examined by transwell assay. The images represent one of three images captured in each group. (B) Quantifications of the migrated and invaded cell numbers of the transwell assay above. (C) Cell migrating speeds were measured by wound healing assay, and the ratios of the gap lengths at 0, 12, and 24 hours to the gap length at 0 hours were quantified and compared. * and ***, p<0.05 and p<0.001, respectively.

Article Snippet: Cell culture and stable cell lines Human bronchial epithelial cells (BEAS-2B) were from ATCC and were cultured in DMEM medium with 10% FBS.

Techniques: Transformation Assay, Migration, Soft Agar Assay, Isolation, Control, Transwell Assay, Wound Healing Assay